cytotoxic effects and osteogenic activity of calcium sulfate with and without recombinant human bone morphogenetic protein 2 and nano-hydroxyapatite adjacent to mg-63 cell line
نویسندگان
چکیده
o bjectives: the aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhbmp2) and nano-hydroxyapatite (n- ha) adjacent to mg-63 cell line. mat erials and methods: to assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (mtt) assay was used. alkaline phosphatase (alp) activity and oste- ogenic activity were evaluated using alizarin red and the von kossa staining and analyzed by one-way anova followed by tukey’s post hoc test. results: the n-ha/cs mixture significantly promoted cell growth in comparison to pure calcium sulfate (cs). moreover, addition of rhbmp2 to cs (p=0.02) and also mixing cs with n-ha led to further increase in extracellular calcium production and alp activity (p=0.03). conclusion: this in vitro study indicates that a scaffold material in combination with an osteoinductive material is effective for bone matrix formation.
منابع مشابه
Cytotoxic Effects and Osteogenic Activity of Calcium Sulfate with and without Recombinant Human Bone Morphogenetic Protein 2 and Nano-Hydroxyapatite Adjacent to MG-63 Cell Line
OBJECTIVES The aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhBMP2) and nano-hydroxyapatite (n-HA) adjacent to MG-63 cell line. MATERIALS AND METHODS To assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay was used. Alkaline phosphatase (ALP) activity and osteogenic activi...
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عنوان ژورنال:
journal of dentistry, tehran university of medical sciencesجلد ۱۲، شماره ۵، صفحات ۳۵۲-۳۶۳
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